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Molecular epidemiology of rotavirus infection in Gauteng and the surrounding areas during the 2010 and 2011 seasons

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dc.contributor.advisor Dewar, John Barr
dc.contributor.author Theron, Elizabeth Maria Charlotte
dc.date.accessioned 2013-05-16T08:03:31Z
dc.date.available 2013-05-16T08:03:31Z
dc.date.issued 2013-05-16
dc.identifier.citation Theron, Elizabeth Maria Charlotte (2013) Molecular epidemiology of rotavirus infection in Gauteng and the surrounding areas during the 2010 and 2011 seasons, University of South Africa, Pretoria, <http://hdl.handle.net/10500/9495> en
dc.identifier.uri http://hdl.handle.net/10500/9495
dc.description.abstract Rotavirus infection causes acute gastroenteritis in children younger than five years of age, and commonly occurring human rotavirus strains include G1 - G4 and G9 associated with P[4], P[6] and P[8]. In this study, of 6050 stool samples collected from a Private Pathology Practice in Pretoria, March 2010 - August 2011, 664 tested positive using Coris test-strips. Of these samples, 752 were retested using EIA and, results showed: Coris sensitivity was 93,7% and specificity 99,8%; the winter epidemic peaked in July of both years; more males and children under 30 months of age were particularly vulnerable to infections. Rotavirus-positive samples from Trichardt, Rustenburg and Middelburg were analysed by PAGE and RT-PCR showing circulating strains as mainly G8P[4] (60%) with short electropherotypes, G12P[8] (66%) with long electropherotypes, and G1P[8] at low incidence in the 2010/2011 seasons. These results suggest additional research to monitor the impacts of recently introduced rotavirus vaccines on changing strain profiles in South African communities en
dc.format.extent 1 online resource (iv, 258 leaves.)
dc.language.iso en en
dc.rights University of South Africa
dc.subject Diarrhoeal disease en
dc.subject Rotavirus en
dc.subject Incidence en
dc.subject Seasonality en
dc.subject Genotypes en
dc.subject Electropherotypes en
dc.subject Pathology practice en
dc.subject Trichardt en
dc.subject Middelburg en
dc.subject Rustenburg en
dc.subject.ddc 616.330096822
dc.subject.lcsh Rotavirus infections -- South Africa -- Gauteng
dc.subject.lcsh Molecular epidemiology -- South Africa -- Gauteng
dc.title Molecular epidemiology of rotavirus infection in Gauteng and the surrounding areas during the 2010 and 2011 seasons en
dc.type Dissertation en
dc.description.department Life and Consumer Sciences en
dc.description.degree M.Sc. (Life Sciences)


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