dc.contributor.advisor |
Dewar, J.B.
|
|
dc.contributor.advisor |
Fouché, Gerda
|
|
dc.contributor.author |
Gonyela, Odwa
|
|
dc.date.accessioned |
2016-09-15T08:46:36Z |
|
dc.date.available |
2016-09-15T08:46:36Z |
|
dc.date.issued |
2016-01 |
|
dc.identifier.citation |
Gonyela, Odwa (2016) A scientific investigation of the immunomodulatory properties of an indigenous plant, sutherlandia frutescens, University of South Africa, Pretoria, <http://hdl.handle.net/10500/21208> |
en |
dc.identifier.uri |
http://hdl.handle.net/10500/21208 |
|
dc.description.abstract |
Traditional medicines prepared from Sutherlandia frutescens are used to manage diseases including HIV and cancer. This study aimed at isolating and identifying biologically active compounds isolated from S. frutescens.
Sutherlandia frutescens plants were collected in Petrusburg and Paarl. Powdered plant material was extracted using ethanol or water and their metabolite composition was compared using UPLC-MS. A novel cycloartane, an acetylated variant of this compound as well as a Sutherlandioside B triterpenoid was isolated and characterised using chromatographic and analytical techniques such as NMR and UPLC-MS.
Preliminary biological studies were conducted to assess the activity of plant extracts on cell toxicity, herpes virus replication and cytokine expression. The results of this study suggest that aqueous extracts from S. frutescens do not appear to be cytotoxic or show anti-herpetic activity, but may activate the immune system by increasing expression of IL-6, IL-10 and TNFα. Further research should be conducted to confirm and optimise these results. |
en |
dc.format.extent |
1 electronic resources ( xi, 90 leaves) : illustrations |
en |
dc.language.iso |
en |
en |
dc.subject |
Sutherlandia frutescens |
en |
dc.subject |
Traditional medicine |
en |
dc.subject |
NMR |
en |
dc.subject |
UPLC-MS |
en |
dc.subject |
HIV |
en |
dc.subject |
AIDS |
en |
dc.subject |
Cytokines |
en |
dc.subject |
Immunomodulation |
en |
dc.subject |
Cytometric bead arrays |
en |
dc.subject |
Flow cytometer |
en |
dc.subject.ddc |
581.6340968 |
|
dc.subject.lcsh |
Medicinal plants -- South Africa -- Petrusburg -- Case studies |
en |
dc.subject.lcsh |
Medicinal plants -- South Africa -- Paarl -- Case studies |
en |
dc.subject.lcsh |
Traditional medicine -- South Africa -- Petrusburg -- Case studies |
en |
dc.subject.lcsh |
Traditional medicine -- South Africa -- Paarl -- Case studies |
en |
dc.subject.lcsh |
Endemic plants -- South Africa -- Petrusburg -- Case studies |
en |
dc.subject.lcsh |
Endemic plants -- South Africa -- Paarl -- Case studies |
en |
dc.title |
A scientific investigation of the immunomodulatory properties of an indigenous plant, Sutherlandia frutescens |
en |
dc.type |
Dissertation |
en |
dc.description.department |
Life and Consumer Sciences |
en |
dc.description.degree |
M. Sc. (Life Science) |
en |