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Detection and molecular identification of Mucorales isolated from spoilt agricultural commodities collected in fresh produce markets in Gauteng province, South Africa

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dc.contributor.advisor Rong, Isabella I. H.
dc.contributor.advisor Jacobs, A.
dc.contributor.advisor Lebelo, S. L.
dc.contributor.author Kwinda, Grace Thiambi
dc.date.accessioned 2015-10-27T07:29:25Z
dc.date.available 2015-10-27T07:29:25Z
dc.date.issued 2014-12
dc.identifier.citation Kwinda, Grace Thiambi (2014) Detection and molecular identification of Mucorales isolated from spoilt agricultural commodities collected in fresh produce markets in Gauteng province, South Africa, University of South Africa, Pretoria, <http://hdl.handle.net/10500/19632> en
dc.identifier.uri http://hdl.handle.net/10500/19632
dc.description.abstract Fruit and vegetables are often spoilt during storage, handling and transportation due to microorganisms. The common spoilage causes are fungi within the order Mucorales, the largest order of the class Zygomycetes. Such spoilage can result in reduced food supplies, poor quality and severe losses to producers and traders. The study was to investigate the type of Mucorales prevalent in various commodities and in a particular market than others. Fifty infected papaya, peaches and strawberries were collected at five occasions from large, medium and small markets. Isolation was done aseptically in a biosafety cabinet. Mucorales were identified morphologically, through culture based tests and molecular techniques. Mucorales isolated are Rhizopus stolonifer, Mucor circinelloides and Mucor racemosus. Mucorales were isolated at a higher rate in samples collected from the small market than other two markets. Spoilage in all three markets is assumed to be influenced by lack of modified temperatures in the storage room. en
dc.format.extent 1 online resource (xvi, 106 leaves) : illustrations (some color)
dc.language.iso en en
dc.subject Culture en
dc.subject Fungi en
dc.subject Market en
dc.subject Morphology en
dc.subject Molecular en
dc.subject Mucorales en
dc.subject Papaya en
dc.subject Peach en
dc.subject Spoilage en
dc.subject Strawberry en
dc.subject.ddc 634.049
dc.subject.lcsh Farm produce -- Storage -- Diseases and injuries
dc.subject.lcsh Microbiology
dc.subject.lcsh Biotechnology
dc.subject.lcsh Produce trade -- South Africa -- Gauteng
dc.subject.lcsh Fruit -- Diseases and pests -- Identification
dc.subject.lcsh Molecular immunology
dc.title Detection and molecular identification of Mucorales isolated from spoilt agricultural commodities collected in fresh produce markets in Gauteng province, South Africa en
dc.type Dissertation en
dc.description.department Life and Consumer Sciences
dc.description.degree M. Sc. (Life Sciences)


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