dc.contributor.advisor |
Dewar, John
|
|
dc.contributor.advisor |
Kenyon, Colin
|
|
dc.contributor.advisor |
Pillay, Pamisha
|
|
dc.contributor.author |
Mbobela, Phindiwe Felicia
|
|
dc.date.accessioned |
2015-10-14T13:06:26Z |
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dc.date.available |
2015-10-14T13:06:26Z |
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dc.date.issued |
2015-01 |
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dc.identifier.citation |
Mbobela, Phindiwe Felicia (2015) Isolation and characterisation of the active phyto-pharmaceutical ingredient from Lobostemon trigonus for use in the development of a microbicide, University of South Africa, Pretoria, <http://hdl.handle.net/10500/19567> |
en |
dc.identifier.uri |
http://hdl.handle.net/10500/19567 |
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dc.description.abstract |
The HIV-1 pandemic affects millions of people worldwide with approximately 70% of those affected residing in sub-Saharan Africa (SSA) relying on traditional medicines for treatment. The key aim of the study was to isolate and characterise an active phyto-pharmaceutical ingredient (API) from L. trigonus for use as a vaginal microbicide.
The aerial parts of L. trigonus were oven-dried at 80°C, ground and then extracted with boiling water for 30 minutes. Aqueous extracts were screened using an HIV-1 neutralization assay in TZM bl cells. Chromatographic and spectroscopic techniques were used to purify, isolate and identify the API.
The API (BP36-117-26464C) was identified as a polymeric macromolecule with IC50 = 0.04 μg/ml against HIV-1 HXB 2 subtype B. This activity is comparable to the ARV drug, enfuvirtide (IC50 = 0.02 μg/ml). The API consists of galacturonic acid polymer and a mixture of seven compounds. Its mode of action may involve inhibiting virus attachment.
The activity of this precipitate (BP36-117-26464C) tested against HIV-1 subtype C pseudovirions and shown to compare favorably with that of enfuvirtide (T20). The water-soluble nature of this API and its mode of action identified it as a potential microbicide. In the current form, the precipitate (API) would be difficult to develop as an oral treatment for HIV, as high-molecular weight agents often have poor bioavailability following oral administration. However, large molecules with potent anti-HIV activity are ideal for topical use and potent development as a microbicide. |
en |
dc.format.extent |
1 online resource (xix, 110 leaves), illustrations (some color) |
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dc.language.iso |
en |
en |
dc.subject |
Lobostemon trigonus |
en |
dc.subject |
Traditional uses |
en |
dc.subject |
Anti-HIV |
en |
dc.subject |
Vaginal microbicide |
en |
dc.subject |
Formulations |
en |
dc.subject |
HIV-1 neutralisation assay |
en |
dc.subject |
Phytochemistry |
en |
dc.subject |
Nuclear magnetic resonance spectroscopy |
en |
dc.subject |
Bornesitol |
en |
dc.subject |
Rosmarinic acid |
en |
dc.subject.ddc |
615.32394 |
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dc.subject.lcsh |
HIV infections |
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dc.subject.lcsh |
AIDS (Disease) |
|
dc.subject.lcsh |
Botanical chemistry -- South Africa |
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dc.subject.lcsh |
Antiviral agents |
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dc.subject.lcsh |
Pharmaceutical chemistry -- South Africa |
|
dc.subject.lcsh |
Medicinal plants |
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dc.title |
Isolation and characterisation of the active phyto-pharmaceutical ingredient from Lobostemon trigonus for use in the development of a microbicide |
en |
dc.type |
Dissertation |
en |
dc.description.department |
Life and Consumer Sciences |
en |
dc.description.degree |
M.Sc (Life Sciences) |
|