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Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents

Show simple item record Sibanda, Timothy Selvarajan, Ramganesh Tekere, Memory 2019-10-10T13:07:36Z 2019-10-10T13:07:36Z 2018-10
dc.identifier.citation Sibanda, T., Selvarajan, R. & Tekere, M. International Microbiology (2019) 22: 181. en
dc.identifier.issn 1618-1905
dc.description Follow the DOI link at the top of the record to access the full-text on the publisher's website
dc.description.abstract This study aimed to analyze the bacterial diversity in carwash effluents and to determine their potential for use in microbial degradation of environmental contaminants. Nine carwash effluent samples were collected for physicochemical and bacterial community diversity analysis using multi-digital probes and 16S rRNA gene amplicon sequencing respectively. The pH of all effluent samples was neutral to slightly alkaline. Oil and grease concentrations ranged from 15.3 to 49.7 mg/L. 16S gene amplicon sequencing of the nine samples produced 45,934-sequence reads, which translated to 13 bacterial phyla, 26 classes, and 43 genera. The most dominant phyla were Proteobacteria, Bacteroidetes, Firmicutes, and Fusobacteria. Canonical correspondence analysis (CCA) showed that the distribution of the phyla Proteobacteria, Bacteroidetes, Acidobacteria, and Verrucomicrobia was influenced by the presence of oil and grease, total petroleum hydrocarbons-gasoline range organics (GRO-TPH), and metals species (Pb, Cu, and Zn). The dominant bacterial genera found in the present study were previously proven to biodegrade hydrocarbons, and their presence in carwash effluents could bode well for in situ natural bioremediation of these contaminated sites. en
dc.description.sponsorship University Of South Africa en
dc.language.iso en en
dc.publisher Springer International Publishing en
dc.subject Carwash en
dc.subject Bacteria en
dc.subject Diversity en
dc.subject Pyrosequencing en
dc.subject Canonical correspondence analysis en
dc.title Targeted 16S rRNA amplicon analysis reveals the diversity of bacterial communities in carwash effluents en
dc.type Article en
dc.description.department Environmental Sciences en

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