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Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)

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dc.contributor.author Fadipe, Victor O
dc.contributor.author Mongalo, Nkoana Ishmael
dc.contributor.author Opoku, Andy R
dc.contributor.author Dikhoba, Preachers M
dc.contributor.author Makhafola, Tshepiso Jan
dc.date.accessioned 2017-07-01T04:10:50Z
dc.date.available 2017-07-01T04:10:50Z
dc.date.issued 2017-06-12
dc.identifier.citation BMC Complementary and Alternative Medicine. 2017 Jun 12;17(1):306
dc.identifier.uri http://dx.doi.org/10.1186/s12906-017-1818-9
dc.identifier.uri http://hdl.handle.net/10500/22764
dc.description.abstract Abstract Background Tuberculosis is counted amongst the most infectious and lethal illnesses worldwide and remains one of the major threats to human health. The aim of the current study was to isolate and characterize anti-mycobacterial compounds present in Curtisia dentata (Burm.f.) C.A.Sm , a medicinal plant reportedly used in the treatment of tuberculosis, stomach ailments and sexually transmitted infections. Methods The bioassay guided principle was followed to isolate the anti-mycobacterial compounds. The crude ethanol extracts of the leaves was partitioned with various solvents four compounds such as β-sitosterol, betulinic acid, ursolic acid and lupeol were successfully isolated. The compounds and their derivatives were evaluated for anti-mycobacterial activity using Microplate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37RV (ATCC 27294). Furthermore, the derivatives were investigated for their toxicity against HepG2 and HEK293 using the MTT assay. Results The methanol fraction had the lowest minimum inhibitory concentration (MIC) of 22.2 μg/ml against the selected Mycobacterium strain when compared to other fractions. Ursolic acid acetate (UAA) was the most active compound with MIC value of 3.4 μg/ml. The derivatives had varying degrees of toxicity, but were generally non-toxic to the selected cell lines. Derivatives also exhibited highest selectivity index and offers a higher safety margin. Conclusions The derivatives had better antimicrobial activity and low cytotoxic effects compared to isolated compounds. These increased their selectivity. It appears that acetylation of both betulinic acid and ursolic acid increased their activity against the selected Mycobacterium species. The results obtained in this study gives a clear indication that Curtisia dentata may serve as major source of new alternative medicines that may be used to treat TB. Furthermore, there is a need to explore the activity of these tested plant against other pathogenic Mycobacterium species.
dc.title Isolation of anti-mycobacterial compounds from Curtisia dentata (Burm.f.) C.A.Sm (Curtisiaceae)
dc.type Journal Article
dc.date.updated 2017-07-01T04:10:50Z
dc.language.rfc3066 en
dc.rights.holder The Author(s).


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