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Antimycobacterial activity and low cytotoxicity of leaf extracts of some African Anacardiaceae tree species

Show simple item record Kayoka, P. N. Eloff, Jacobus N. Kabongo-Kayoka, Prudence N. Obi, Chikwelu L. McGaw, Lyndy J. 2016-11-07T12:18:05Z 2016-11-07T12:18:05Z 2016-09-13
dc.identifier.citation Kabongo‐Kayoka, Prudence N., et al. "Antimycobacterial Activity and Low Cytotoxicity of Leaf Extracts of Some African Anacardiaceae Tree Species." Phytotherapy Research (2016). en
dc.identifier.issn 0951-418X
dc.description.abstract Treatment of tuberculosis is a challenge due to multi and extremely drug resistant strains of Mycobacterium tuberculosis. Plant species contain antimicrobial compounds that may lead to new anti-TB drugs. Previous screening of some tree species from the Anacardiaceae family revealed the presence of antimicrobial activity, justifying further investigations. Leaf extracts of 15 Anacardiaceae tree species were screened for antimycobacterial activity using a twofold serial microdilution assay against the pathogenic Mycobacterium bovis and multidrug resistant M. tuberculosis and rapidly growing mycobacteria, Mycobacterium smegmatis, Mycobacterium fortuitum and Mycobacterium aurum. The vaccine strain, M. bovis and an avirulent strain, H37Ra M. tuberculosis, were also used. Cytotoxicity was assessed using a colorimetric assay against Vero kidney, human hepatoma and murine macrophage cells. Four out of 15 crude acetone extracts showed significant antimycobacterial activity with MIC varying from 50 to 100μg/mL. Searsia undulata had the highest activity against most mycobacteria, followed by Protorhus longifolia. M. fortuitum was the strongest predictor of activity against MDR-TB (correlation coefficient=0.65). Bioautography against M. aurum and M. fortuitum worked well as indicators of the Rf values of active compounds yielding strong zones of inhibition. The leaf extracts of S. undulata and P. longifolia had more than 10 different antimycobacterial compounds and had low cytotoxicity with LC50 values above 100μg/ml. en
dc.description.sponsorship UNISA National Research Foundation (NRF) en
dc.language.iso en en
dc.publisher Wiley en
dc.subject Anacardiaceae en
dc.subject MDR-M en
dc.subject tuberculosis en
dc.subject antimycobacterial en
dc.subject cytotoxicity en
dc.subject selectivity en
dc.title Antimycobacterial activity and low cytotoxicity of leaf extracts of some African Anacardiaceae tree species en
dc.title.alternative Antimycobacterial and cytotoxicity of Anacardiaceae tree species en
dc.type Article en
dc.description.department Agriculture, Animal Health and Human Ecology en

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