Institutional Repository

Characterization and Molecular Epidemiology of Rotavirus Strains Recovered in NorthernSouth Africa during 2003–2006 Pretoria,

Show simple item record Seheri, L.M. Dewar, J.B. Nemarude, A.L. Esona, M. Page, N. Geyer, A. Bos, P. Steele, A.D. 2014-08-27T13:41:12Z 2014-08-27T13:41:12Z 2010-09
dc.identifier.citation DOI: 10.1086/653559 en
dc.identifier.issn 0022-1899 (online)
dc.description Presented in part: 8th International Rotavirus Symposium, Istanbul, June 2008; and 4th African Rotavirus Symposium, Mauritius, July 2008. en
dc.description.abstract Rotavirus infection is the most common cause of severe dehydrating gastroenteritis in infants and young children and remains a significant clinical problem worldwide. The severity and the burden of rotavirus disease could be reduced through the implementation of an effective vaccine. The aim of this study was to characterize rotavirus strains circulating in the local community as part of an ongoing hospital burden of disease study when a G1P[8] rotavirus vaccine candidate was being evaluated in the same community. From 2003 through 2006, 729 rotavirus-positive stool specimens were collected from children under !5 years of age who were treated for diarrhea at Dr George Mukhari Hospital, Ga-Rankuwa, South Africa. Molecular haracterization of the strains was performed by polyacrylamide gel electrophoresis and genotyping of the VP4 and VP7 alleles using well-established seminested multiplex reverse-transcription polymerase chain reaction methods. In 2003, 62% of strains exhibited the short rotavirus electropherotype, and the most common rotavirus strain was G2P[4]. In subsequent years, predominant rotavirus strains included G1P[8] and G1P[6] in 2004, G3P[8] and G3P[6]in 2005, and G1P[8] in 2006. For the 4 years of the study, rotavirus strains with P[6] genotype were detected in 25% of all rotavirus-positive specimens. In addition, unusual G12P[6] and G8 strains were detected at a low frequency. These results reflect the diversity of rotavirus strains circulating in South African communities. en
dc.description.sponsorship Financial support: World Health Organization (V27/181/113); the Norwegian Programme for Development, Research and Higher Education Research Grant (PRO 48/2002); the Rotavirus Vaccine Program, PATH (GAV.1142-01-07211-SPS); the Poliomyelitis Research Foundation (PRF 04/06); and the South African Medical Research Council. Potential conflicts of interest: none reported. Supplement sponsorship: This article is part of a supplement entitled “Rotavirus Infection in Africa: Epidemiology, Burden of Disease, and Strain Diversity,” which was prepared as a project of the Rotavirus Vaccine Program, a partnership among PATH, the World Health Organization, and the US Centers for Disease Control and Prevention, and was funded in full or in part by the GAVI Alliance. en
dc.language.iso en en
dc.publisher The University of Chicago Press en
dc.subject Rotavirus infection en
dc.subject dehydrating gastroenteritis en
dc.subject infants en
dc.subject young children en
dc.subject G1P[8] rotavirus vaccine en
dc.subject Dr George Mukhari Hospital en
dc.subject Ga-Rankuwa en
dc.subject rotavirus strains en
dc.title Characterization and Molecular Epidemiology of Rotavirus Strains Recovered in NorthernSouth Africa during 2003–2006 Pretoria, en
dc.type Article en

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UnisaIR


My Account